Alternative Thioglycollate Medium
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TMKH 010 – Alternative Thioglycollate Medium (USP)
Intended Use
For sterility testing of biological products.
Product Summary and Explanation
Alternative Thioglycollate Medium is formulated as described in N.I.H. Memorandum, U.S. Pharmacopoeia. This medium is recommended for sterility testing for detecting the presence of viable forms of microorganisms in or on pharmaceutical preparations.
Composition
Ingredients | Gms / Ltr |
---|---|
Pancreatic digest of casein | 15.000 |
Yeast extract | 5.000 |
Dextrose monohydrate | 5.500 |
Sodium chloride | 2.500 |
L-Cystine | 0.500 |
Sodium thioglycollate | 0.500 |
Principle
Absence of agar makes it suitable for testing viscous materials and devices having tubes with small lumina. Pancreatic digest of casein, yeast extract, dextrose monohydrate, L - cystine provides nitrogenous and carbonaceous compounds, vitamin B complex, trace elements and other essential growth nutrients. Sodium Thioglycollate and L-cystine lower the oxidation-reduction potential of the medium by removing oxygen radicals and thus preventing the accumulation of peroxides that can be toxic to some organisms. The sulfhydryl groups of these compounds also neutralize the antibacterial effect of mercurial preservatives with heavy metals. Dextrose is the fermentable carbohydrate energy source, and Sodium Chloride maintains the osmotic balance of the medium.