Sabouraud Chloramphenicol Agar Plate
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TMP 010GT - Sabouraud Chloramphenicol Agar Plate (γ- IRRADIATED)(TRIPLE PACK)
Intended Use
For selective cultivation of yeasts and molds.
Product Summary and Explanation
Sabouraud Chloramphenicol Agar is used for the propagation of yeast and molds, particularly the parasitic fungi concerned with skin and scalp lesions. Sabouraud Chloramphenicol Agar was formulated by Scientist “Sabouraud”. The medium is often used with antibiotics such as Chloramphenicol for the isolation of pathogenic fungi from materials containing large numbers of fungi or bacteria.
Composition
Ingredients | Gms / Ltr |
---|---|
Dextrose | 40.000 |
Agar | 15.000 |
Casein enzymic hydrolysate | 5.000 |
Peptic digest of animal tissue | 5.000 |
Chloramphenicol | 0.050 |
Principle
The medium contains casein enzymic hydrolysate and peptic digest of animal tissue which provides nitrogen, vitamins, minerals, amino acids and growth factors. Dextrose serves as the energy and carbon source for fungi. Chloramphenicol inhibits a wide range of gram-positive and gram-negative bacteria which makes the medium selective for fungi. Agar is a solidifying agent.
The low pH favors fungal growth and inhibits contaminating bacteria from clinical specimens. For isolation of fungi from contaminated specimens, a selective medium should be inoculated simultaneously.